General hints

When fishing for crystals in the cryoprotectant, have the microscope on fairly low magnification so that the whole drop is visible and the depth of field is larger than on high magnification
Also when fishing, rest the fourth finger of the ‘fishing’ hand on the edge of the crystallisation tray to steady your hand and act as an anchor point
When manipulating crystals and vials under liquid nitrogen, wait until the liquid has stopped bubbling and is calm before trying to see where the objects are under the liquid
Also, it is easier to move only one hand at a time and rest the side of the stationary hand on the edge of the Dewar top